Your browser doesn't support javascript.
loading
Functionally distinct subgroups of oligodendrocyte precursor cells integrate neural activity and execute myelin formation.
Marisca, Roberta; Hoche, Tobias; Agirre, Eneritz; Hoodless, Laura Jane; Barkey, Wenke; Auer, Franziska; Castelo-Branco, Gonçalo; Czopka, Tim.
Afiliação
  • Marisca R; Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  • Hoche T; Graduate School of Systemic Neurosciences, Ludwig-Maximilian University of Munich, Planegg-Martinsried, Germany.
  • Agirre E; Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  • Hoodless LJ; Laboratory of Molecular Neurobiology, Department Medical Biochemistry and Biophysics, Biomedicum, Karolinska Institutet, Stockholm, Sweden.
  • Barkey W; Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  • Auer F; Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  • Castelo-Branco G; Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany.
  • Czopka T; Graduate School of Systemic Neurosciences, Ludwig-Maximilian University of Munich, Planegg-Martinsried, Germany.
Nat Neurosci ; 23(3): 363-374, 2020 03.
Article em En | MEDLINE | ID: mdl-32066987
ABSTRACT
Recent reports have revealed that oligodendrocyte precursor cells (OPCs) are heterogeneous. It remains unclear whether such heterogeneity reflects different subtypes of cells with distinct functions or instead reflects transiently acquired states of cells with the same function. By integrating lineage formation of individual OPC clones, single-cell transcriptomics, calcium imaging and neural activity manipulation, we show that OPCs in the zebrafish spinal cord can be divided into two functionally distinct groups. One subgroup forms elaborate networks of processes and exhibits a high degree of calcium signaling, but infrequently differentiates despite contact with permissive axons. Instead, these OPCs divide in an activity- and calcium-dependent manner to produce another subgroup, with higher process motility and less calcium signaling and that readily differentiates. Our data show that OPC subgroups are functionally diverse in their response to neurons and that activity regulates the proliferation of a subset of OPCs that is distinct from the cells that generate differentiated oligodendrocytes.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Precursoras de Oligodendrócitos / Bainha de Mielina Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Precursoras de Oligodendrócitos / Bainha de Mielina Idioma: En Ano de publicação: 2020 Tipo de documento: Article