RNA-based fluorescent biosensors for live cell imaging of small molecules and RNAs.
Curr Opin Biotechnol
; 63: 157-166, 2020 06.
Article
em En
| MEDLINE
| ID: mdl-32086101
ABSTRACT
Genetically encodable fluorescent biosensors provide spatiotemporal information on their target analytes in a label-free manner, which has enabled the study of cell biology and signaling in living cells. Over the past three decades, fueled by the development of a wide palette of fluorescent proteins, protein-based fluorescent biosensors against a broad array of targets have been developed. Recently, with the development of fluorogenic RNA aptamer-dye pairs that function in live cells, RNA-based fluorescent (RBF) biosensors have emerged as a complementary class of biosensors. Here we review the current state-of-the-art for fluorogenic RNA aptamers and RBF biosensors for imaging small molecules and RNAs, and highlight some emerging opportunities.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Aptâmeros de Nucleotídeos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article