Vesicle Clustering in a Living Synapse Depends on a Synapsin Region that Mediates Phase Separation.
Cell Rep
; 30(8): 2594-2602.e3, 2020 02 25.
Article
em En
| MEDLINE
| ID: mdl-32101738
ABSTRACT
Liquid-liquid phase separation is an increasingly recognized mechanism for compartmentalization in cells. Recent in vitro studies suggest that this organizational principle may apply to synaptic vesicle clusters. Here we test this possibility by performing microinjections at the living lamprey giant reticulospinal synapse. Axons are maintained at rest to examine whether reagents introduced into the cytosol enter a putative liquid phase to disrupt critical protein-protein interactions. Compounds that perturb the intrinsically disordered region of synapsin, which is critical for liquid phase organization in vitro, cause dispersion of synaptic vesicles from resting clusters. Reagents that perturb SH3 domain interactions with synapsin are ineffective at rest. Our results indicate that synaptic vesicles at a living central synapse are organized as a distinct liquid phase maintained by interactions via the intrinsically disordered region of synapsin.
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Base de dados:
MEDLINE
Assunto principal:
Vesículas Sinápticas
/
Sinapsinas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article