Role of autophagy in Puromycin Aminonucleoside-induced podocyte apoptosis.
J Recept Signal Transduct Res
; 40(3): 273-280, 2020 Jun.
Article
em En
| MEDLINE
| ID: mdl-32102592
ABSTRACT
Objective:
The aim of our study is to investigate the relationship between podocyte autophagy and apoptosis induced by Puromycin Aminonucleoside (PAN) and to clarify its mechanism.Methods:
Podocytes were cultured in vitro. The apoptosis rates of each group were detected using flow cytometry. The expression of LC3-II protein and changes in distribution were detected through laser scanning confocal microscope, and the western blot protocol was employed for detection of protein expression of LC3-II. The autophagosomes were detected by transmission electron microscopy.Results:
In this study, We found that autophagosome increased followed by apoptosis after podocyte injury. Furthermore, we conformed that the activation of autophagy could inhibit the apoptosis to alleviate the injury of podocyte at an early stage.Conclusions:
Autophagy occurred earlier before apoptosis and autophagy mediated podocyte apoptosis induced by PAN. These findings indicate that autophagy may become a novel therapeutic target for the treatment of podocyte injury and proteinuria in the future.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Puromicina Aminonucleosídeo
/
Apoptose
/
Podócitos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article