Equol Inhibits Mushroom Tyrosinase in Vitro through Tight Binding.
Biol Pharm Bull
; 43(3): 550-553, 2020.
Article
em En
| MEDLINE
| ID: mdl-32115514
ABSTRACT
Equol, an intestinal metabolite of daidzein, inhibited more potently mushroom tyrosinase in vitro than other inhibitors, genistein and kojic acid. We investigated the mechanism underlying tyrosinase inhibition by equol. Treating racemic equol with tyrosinase produced 3'-hydroxyequol. Because the optical activity of the product showed <25% enantiomeric excess, the reaction was not highly stereospecific. Using enzyme-linked immunosorbent assays with an anti-equol monoclonal antibody, we observed that equol bound to pre-coated tyrosinase in a dose-dependent manner. Our results suggested the formation of a stable equol-tyrosinase complex.
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Base de dados:
MEDLINE
Assunto principal:
Monofenol Mono-Oxigenase
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Agaricales
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Equol
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article