Enhancing glutaric acid production in Escherichia coli by uptake of malonic acid.
J Ind Microbiol Biotechnol
; 47(3): 311-318, 2020 Mar.
Article
em En
| MEDLINE
| ID: mdl-32140931
ABSTRACT
Glutaric acid is an important organic acid applied widely in different fields. Most previous researches have focused on the production of glutaric acid in various strains using the 5-aminovaleric acid (AMV) or pentenoic acid synthesis pathways. We previously utilized a five-step reversed adipic acid degradation pathway (RADP) in Escherichia coli BL21 (DE3) to construct strain Bgl146. Herein, we found that malonyl-CoA was strictly limited in this strain, and increasing its abundance could improve glutaric acid production. We, therefore, constructed a malonic acid uptake pathway in E. coli using matB (malonic acid synthetase) and matC (malonic acid carrier protein) from Clover rhizobia. The titer of glutaric acid was improved by 2.1-fold and 1.45-fold, respectively, reaching 0.56 g/L and 4.35 g/L in shake flask and batch fermentation following addition of malonic acid. Finally, the highest titer of glutaric acid was 6.3 g/L in fed-batch fermentation at optimized fermentation conditions.
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Base de dados:
MEDLINE
Assunto principal:
Escherichia coli
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Glutaratos
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Malonatos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article