Duck PIAS2 negatively regulates RIG-I mediated IFN-ß production by interacting with IRF7.
Dev Comp Immunol
; 108: 103664, 2020 07.
Article
em En
| MEDLINE
| ID: mdl-32151676
ABSTRACT
The protein inhibitor of activated STAT (PIAS) proteins are important signal transduction modulator family and regulate the innate immune signaling pathway induced by certain transcription factors, including NF-κB, IRF3, and JAK/STAT. The PIAS protein mechanism that regulates innate immune response in mammals has been well described in the literature; however, whether the PIAS gene exists in ducks as well as the role of PIAS in duck IFN-ß expression is still unclear. Here, we cloned duck PIAS (duPIAS), finding PIAS2 could repress IFN-ß production. DuPIAS2 contains SAP-PINIT-RLD-S/T characteristic domains, and its overexpression could inhibit virus-induced IFN-ß promoter activation. Moreover, duPIAS2 interacts with duck interferon regulatory factor 7 (IRF7) and inhibits IFN-ß promoter activation induced by duck IRF7. Additionally, its inhibitory function does not rely on its SUMO E3 ligase activity but rather its C-terminal portion. The above results demonstrate that duPIAS2 is a repressor of IFN-ß production induced by duck IRF7.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
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Patos
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Fator Regulador 7 de Interferon
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Proteínas Inibidoras de STAT Ativados
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article