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Discordant Activity of Kaempferol Towards Dengue Virus and Japanese Encephalitis Virus.
Care, Chit; Sornjai, Wannapa; Jaratsittisin, Janejira; Hitakarun, Atitaya; Wikan, Nitwara; Triwitayakorn, Kanokporn; Smith, Duncan R.
Afiliação
  • Care C; Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand.
  • Sornjai W; Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand.
  • Jaratsittisin J; Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand.
  • Hitakarun A; Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand.
  • Wikan N; Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand.
  • Triwitayakorn K; Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand.
  • Smith DR; Institute of Molecular Biosciences, Mahidol University, Salaya 73170, Thailand.
Molecules ; 25(5)2020 Mar 10.
Article em En | MEDLINE | ID: mdl-32164193
ABSTRACT
Kaempferol, a plant-derived flavonoid, has been reported to have activity against Japanese encephalitis virus (JEV) in BHK-21 cells. To determine the broader utility of this compound, we initially evaluated the activity of kaempferol against JEV and dengue virus (DENV) in HEK293T/17 cells. Results showed no significant antiviral activity against either virus. We subsequently investigated the activity of kaempferol against both JEV and DENV in BHK-21 cells. Results showed a significant inhibition of JEV infection but, surprisingly, a significant enhancement of DENV infection. The effect of kaempferol on both host protein expression and transcription was investigated and both transcriptional and translational inhibitory effects were observed, although a more marked effect was observed on host cell protein expression. Markedly, while GRP78 was increased in DENV infected cells treated with kaempferol, it was not increased in JEV infected cells treated with kaempferol. These results show that cellular alteration induced by one compound can have opposite effects on viruses from the same family, suggesting the presence of distinct replication strategies for these two viruses.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vírus da Dengue / Quempferóis / Vírus da Encefalite Japonesa (Espécie) Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vírus da Dengue / Quempferóis / Vírus da Encefalite Japonesa (Espécie) Idioma: En Ano de publicação: 2020 Tipo de documento: Article