A Highly Selective Chemical Probe for Activin Receptor-like Kinases ALK4 and ALK5.
ACS Chem Biol
; 15(4): 862-870, 2020 04 17.
Article
em En
| MEDLINE
| ID: mdl-32176847
ABSTRACT
The transforming growth factor beta-receptor I/activin receptor-like kinase 5 (TGFBR1/ALK5) and its close homologue ALK4 are receptor protein kinases associated with the development of diverse diseases, including cancer, fibrosis, heart diseases, and dysfunctional immune response. Therefore, ALK4/5 are among the most studied kinases, and several inhibitors have been developed. However, current commercially available inhibitors either lack selectivity or have not been comprehensively characterized, limiting their value for studying ALK4/5 function in cellular systems. To this end, we report the characterization of the 2-oxo-imidazopyridine, TP-008, a potent chemical probe with dual activity for ALK4 and ALK5 as well as the development of a matching negative control compound. TP-008 has excellent cellular potency and strongly abrogates phosphorylation of the substrate SMAD2 (mothers against decapentaplegic homologue 2). Thus, this chemical probe offers an excellent tool for mechanistic studies on the ALK4/5 signaling pathway and the contribution of these targets to disease.
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Base de dados:
MEDLINE
Assunto principal:
Piridinas
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Receptores de Ativinas Tipo I
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Inibidores de Proteínas Quinases
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Receptor do Fator de Crescimento Transformador beta Tipo I
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Imidazóis
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article