An Enzymatic Flow-Based Preparative Route to Vidarabine.
Molecules
; 25(5)2020 Mar 09.
Article
em En
| MEDLINE
| ID: mdl-32182773
ABSTRACT
The bi-enzymatic synthesis of the antiviral drug vidarabine (arabinosyladenine, ara-A), catalyzed by uridine phosphorylase from Clostridium perfringens (CpUP) and a purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP), was re-designed under continuous-flow conditions. Glyoxyl-agarose and EziGTM1 (Opal) were used as immobilization carriers for carrying out this preparative biotransformation. Upon setting-up reaction parameters (substrate concentration and molar ratio, temperature, pressure, residence time), 1 g of vidarabine was obtained in 55% isolated yield and >99% purity by simply running the flow reactor for 1 week and then collecting (by filtration) the nucleoside precipitated out of the exiting flow. Taking into account the substrate specificity of CpUP and AhPNP, the results obtained pave the way to the use of the CpUP/AhPNP-based bioreactor for the preparation of other purine nucleosides.
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Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Vidarabina
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Purina-Núcleosídeo Fosforilase
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Enzimas Imobilizadas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article