Changes in Microbiota Profiles After Prolonged Frozen Storage of Stool Suspensions.

Dorsaz, Stéphane; Charretier, Yannick; Girard, Myriam; Gaïa, Nadia; Leo, Stefano; Schrenzel, Jacques; Harbarth, Stephan; Huttner, Benedikt; Lazarevic, Vladimir

Dorsaz, Stéphane; Charretier, Yannick; Girard, Myriam; Gaïa, Nadia; Leo, Stefano; Schrenzel, Jacques; Harbarth, Stephan; Huttner, Benedikt; Lazarevic, Vladimir.

Afiliação

Dorsaz S; Genomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Charretier Y; Genomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Girard M; Genomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Gaïa N; Genomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Leo S; Genomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Schrenzel J; Genomic Research Laboratory, Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Harbarth S; Bacteriology Laboratory, Division of Laboratory Medicine, Department of Diagnostics, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Huttner B; Infection Control Program, Division of Infectious Diseases, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
Lazarevic V; Infection Control Program, Division of Infectious Diseases, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.

Front Cell Infect Microbiol ; 10: 77, 2020.

Article em En | MEDLINE | ID: mdl-32185143

Assuntos

Bactérias/classificação; Bacteroidetes/genética; Fezes/microbiologia; Firmicutes/genética; Microbiota; Bactérias/isolamento & purificação; Bacteroidetes/isolamento & purificação; Criopreservação/métodos; DNA Bacteriano/genética; Transplante de Microbiota Fecal; Firmicutes/isolamento & purificação; Humanos; Reação em Cadeia da Polimerase; RNA Ribossômico 16S/genética; Manejo de Espécimes/métodos; Suspensões; Fatores de Tempo

Palavras-chave

DNA extraction; fecal microbiota transplantation; long-term frozen storage; microbiota; stool; taxonomic profiling

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bactérias / Bacteroidetes / Fezes / Microbiota / Firmicutes Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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