Conversion of human urine-derived cells into neuron-like cells by small molecules.
Mol Biol Rep
; 47(4): 2713-2722, 2020 Apr.
Article
em En
| MEDLINE
| ID: mdl-32185687
ABSTRACT
Neural cell transplantation is an effective way for treatment of neurological diseases. However, the absence of transplantable human neurons remains a barrier for clinical therapies. Human urine-derived cells, namely renal cells and urine stem cells, have become a good source of cells for reprogramming or trans-differentiation research. Here, we show that human urine-derived cells can be partially converted into neuron-like cells by applying a cocktail of small molecules. Gene expression analysis has shown that these induced cells expressed some neuron-specific genes, and a proportion of the cells are GABAergic neurons. Moreover, whole-cell patch clamping recording has shown that some induced cells have neuron-specific voltage gated Na+ and K+ currents but have failed to generate Ca2+ currents and action potentials. Taken together, these results suggest that induced neuronal cells from human urine-derived cells may be useful for neurological disease modelling, drug screening and cell therapies.
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Base de dados:
MEDLINE
Assunto principal:
Urina
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Técnicas de Cultura de Células
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Células-Tronco Neurais
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Neurônios
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article