Your browser doesn't support javascript.
loading
Generation of twelve induced pluripotent stem cell lines from two healthy controls and two patients with sporadic amyotrophic lateral sclerosis.
Yang, Meimei; Liu, Min; Ding, Yicheng; Vajda, Alice; Ma, Jun; Cui, Huixian; O'Brien, Timothy; Henshall, David; Hardiman, Orla; Shen, Sanbing.
Afiliação
  • Yang M; Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland.
  • Liu M; Department of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
  • Ding Y; Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland.
  • Vajda A; Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland.
  • Ma J; Hebei Medical University-NUI Galway Stem Cell Research Center, Hebei Research Center for Stem Cell Medical Translational Engineering, Human Anatomy Department, Hebei Medical University, Hebei 050017, China.
  • Cui H; Hebei Medical University-NUI Galway Stem Cell Research Center, Hebei Research Center for Stem Cell Medical Translational Engineering, Human Anatomy Department, Hebei Medical University, Hebei 050017, China.
  • O'Brien T; Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland; Hebei Medical University-NUI Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China.
  • Henshall D; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland.
  • Hardiman O; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland; Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland. Electronic address: orla@h
  • Shen S; Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland (NUI) Galway, Galway, Ireland; FutureNeuro, The SFI Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, D02, Dublin, Ireland; Hebei Medical University-NUI Galway
Stem Cell Res ; 44: 101752, 2020 04.
Article em En | MEDLINE | ID: mdl-32208303
ABSTRACT
The majority of amyotrophic lateral sclerosis are sporadic (sALS) with no familial history or known genetic association, therefore a large cohort of disease models are required to identify common mechanisms or to test therapeutic interventions. Here we generated twelve induced pluripotent stem cell (iPSC) lines from human dermal fibroblasts of two healthy individuals and two sALS patients lacking common ALS mutations, using non-integrational Sendai virus expressing reprogramming factors OCT3/4, KLF4, SOX2 and c-MYC. The iPSC lines highly expressed pluripotency markers could be spontaneously differentiated into three embryonic germ layers, with no gross chromosomal aberrations or specific copy number variations.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Esclerose Lateral Amiotrófica Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Esclerose Lateral Amiotrófica Idioma: En Ano de publicação: 2020 Tipo de documento: Article