LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p.
Aging (Albany NY)
; 12(7): 5625-5639, 2020 03 31.
Article
em En
| MEDLINE
| ID: mdl-32229708
ABSTRACT
Although many long non-coding RNAs (lncRNAs) have been identified in muscle, some of their physiological functions and regulatory mechanisms remain elusive. Here we report the functional identification and characterization of a novel lncRNA 2310043L19Rik (lnc-231), which is highly expressed in muscle. The expression level of lnc-231 in skeletal muscle of young mice is higher than that in aged mice. Functional analysis showed that overexpression of lnc-231 restrained differentiation and promoted proliferation of myoblast, while inhibition of lnc-231 revealed completely opposite effects in vitro. RNA molecules of lnc-231 acted mechanistically as competing endogenous RNAs (ceRNA) to target miR-125a-5p, whereas miR-125a-5p binds to the 3'-UTR of E2F3 mRNA to inhibit its function. Collectively, lncRNA 2310043L19Rik promotes proliferation and inhibits differentiation of myoblast cells by attenuating the function of miR-125a-5p.
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Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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Músculo Esquelético
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Mioblastos
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MicroRNAs
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Proliferação de Células
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article