A Silyl Ether Based Fluorescent Probe for Rapid Monitoring of Endogenous Peroxynitrite Concentration and Imaging in Living Cells through Multicolor Emission.
Chempluschem
; 85(4): 684-688, 2020 04.
Article
em En
| MEDLINE
| ID: mdl-32253835
ABSTRACT
The peroxynitrite ion (ONOO- ) has important roles in many biological processes. We have developed a multicolor ONOO- -sensing probe (SiONNOH) that undergoes deprotonation and desilylation processes, which result in several changes in the emission wavelengths. In response to different concentrations of ONOO- , the probe exhibits fluorescence changes from pink (595â
nm at 2â
eq. ONOO- ) to green (540â
nm at 6â
eq. ONOO- ) via orange (3â
eq. ONOO- ) and yellow (4â
eq. ONOO- ) under physiological conditions until no fluorescence signal is observed after ONOO- is completely eliminated by lipoic acid. The probe shows the high selectivity for ONOO- and the limit of detection is calculated to be 1.27â
µM. Moreover, the probe shows the capacity to monitor the concentration ranges of ONOO- through multicolor fluorescence in living cells, which will greatly facilitate the rapid detection of ONOO- concentration ranges by the naked eye under a UV light without any precision instrumentation.
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Base de dados:
MEDLINE
Assunto principal:
Silanos
/
Cor
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Ácido Peroxinitroso
/
Éteres
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Imagem Óptica
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article