Your browser doesn't support javascript.
loading
Acrocomia aculeata (Jacq.) Lodd. ex Mart. Leaves Increase SIRT1 Levels and Improve Stress Resistance.
Monteiro-Alfredo, Tamaeh; Matafome, Paulo; Iacia, Bianca Pancoti; Antunes, Kátia Ávila; Dos Santos, Jéssica Maurino; da Silva Melo da Cunha, Janielle; Oliveira, Sara; Oliveira, Alex Santos; Campos, Jaqueline Ferreira; Magalhães, Mariana; Cabral, Célia; Seiça, Raquel; Cardoso, Cláudia Andrea Lima; de Oliveira, Caio Fernando Ramalho; Dos Santos, Edson Lucas; de Picoli Souza, Kely.
Afiliação
  • Monteiro-Alfredo T; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
  • Matafome P; Institute of Physiology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
  • Iacia BP; Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine; CNC.IBILI Consortium & CIBB Consortium, University of Coimbra, 3000-548 Coimbra, Portugal.
  • Antunes KÁ; Institute of Physiology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
  • Dos Santos JM; Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine; CNC.IBILI Consortium & CIBB Consortium, University of Coimbra, 3000-548 Coimbra, Portugal.
  • da Silva Melo da Cunha J; Department of Complementary Sciences, Instituto Politécnico de Coimbra, Coimbra Health School (ESTeSC), Coimbra, Portugal.
  • Oliveira S; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
  • Oliveira AS; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
  • Campos JF; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
  • Magalhães M; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
  • Cabral C; Institute of Physiology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
  • Seiça R; Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine; CNC.IBILI Consortium & CIBB Consortium, University of Coimbra, 3000-548 Coimbra, Portugal.
  • Cardoso CAL; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
  • de Oliveira CFR; Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
  • Dos Santos EL; Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine; CNC.IBILI Consortium & CIBB Consortium, University of Coimbra, 3000-548 Coimbra, Portugal.
  • de Picoli Souza K; Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine; CNC.IBILI Consortium & CIBB Consortium, University of Coimbra, 3000-548 Coimbra, Portugal.
Oxid Med Cell Longev ; 2020: 5238650, 2020.
Article em En | MEDLINE | ID: mdl-32256951
ABSTRACT
Oxidative stress is a metabolic disorder linked with several chronic diseases, and this condition can be improved by natural antioxidants. The fruit pulp of the palm Acrocomia aculeata (Jacq.) Lodd. ex Mart. is widely used in the treatment of various illnesses, but as far as we know, there are no reports regarding the properties of its leaves. Thus, we aimed to evaluate the antioxidant activity of A. aculeata leaf extracts obtained with water (EA-Aa), ethanol (EE-Aa), and methanol (EM-Aa) solvents. The extracts were chemically characterized, and their antioxidant activity was assessed through the scavenging of the free radicals DPPH and ABTS. EE-Aa and EM-Aa showed the highest amounts of phenolic compounds and free radical scavenging activity. However, EA-Aa was more efficient to protect human erythrocytes against AAPH-induced hemolysis and lipid peroxidation. Thus, we further show the antioxidant effect of EA-Aa in preventing AAPH-induced protein oxidation, H2O2-induced DNA fragmentation, and ROS generation in Cos-7 cells. Increased levels of Sirt1, catalase, and activation of ERK and Nrf2 were observed in Cos-7 treated with EA-Aa. We also verify increased survival in nematodes C. elegans, when induced to the oxidative condition by Juglone. Therefore, our results showed a typical chemical composition of plants for all extracts, but the diversity of compounds presented in EA-Aa is involved in the lower toxicity and antioxidant properties provided to the macromolecules tested, proteins, DNA, and lipids. This protective effect also proven in Cos-7 and in C. elegans was probably due to the activation of the Sirt1/Nrf2 pathway. Altogether, the low toxicity and the antioxidant properties of EA-Aa showed in all the experimental models support its further use in the treatment of oxidative stress-related diseases.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Folhas de Planta / Sirtuína 1 / Frutas Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Folhas de Planta / Sirtuína 1 / Frutas Idioma: En Ano de publicação: 2020 Tipo de documento: Article