Engineering Prokaryotic Transcriptional Activator XylR as a Xylose-Inducible Biosensor for Transcription Activation in Yeast.

Wei, Wenping; Shang, Yanzhe; Zhang, Ping; Liu, Yong; You, Di; Yin, Bincheng; Ye, Bangce

Wei, Wenping; Shang, Yanzhe; Zhang, Ping; Liu, Yong; You, Di; Yin, Bincheng; Ye, Bangce.

Afiliação

Wei W; Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Shang Y; Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Zhang P; Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Liu Y; Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
You D; Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Yin B; Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Ye B; Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

ACS Synth Biol ; 9(5): 1022-1029, 2020 05 15.

Article em En | MEDLINE | ID: mdl-32268060

Assuntos

Técnicas Biossensoriais/métodos; Proteínas de Escherichia coli/genética; Engenharia Metabólica; Saccharomyces cerevisiae/metabolismo; Fatores de Transcrição/genética; Ativação Transcricional/efeitos dos fármacos; Xilose/farmacologia; Escherichia coli/genética; Proteínas Fúngicas/genética; Proteínas Luminescentes/genética; Proteínas Luminescentes/metabolismo; Regiões Promotoras Genéticas; Saccharomyces cerevisiae/genética; Yarrowia/genética; Proteína Vermelha Fluorescente

Palavras-chave

Escherichia coli XylR; Yarrowia liapolytica; hybrid promoter; transcription activator VPRHX; xylose utilization; xylose-inducible biosensor

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Fatores de Transcrição / Xilose / Técnicas Biossensoriais / Ativação Transcricional / Proteínas de Escherichia coli / Engenharia Metabólica Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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