Genital Shedding of Human Immunodeficiency Virus Type-1 (HIV) When Antiretroviral Therapy Suppresses HIV Replication in the Plasma.
J Infect Dis
; 222(5): 777-786, 2020 08 04.
Article
em En
| MEDLINE
| ID: mdl-32274499
ABSTRACT
BACKGROUND:
During antiretroviral treatment (ART) with plasma HIV RNA below the limit of quantification, HIV RNA can be detected in genital or rectal secretions, termed discordant shedding (DS). We hypothesized that proliferating cells produce virions without HIV replication.METHODS:
ART-naive Peruvians initiating ART were observed for DS over 2 years. HIV env and pol genomes were amplified from DS. Antiretrovirals and cytokines/chemokines concentrations were compared at DS and control time points.RESULTS:
Eighty-two participants had ART suppression. DS was detected in 24/82 (29%)participants:
13/253 (5%) cervicovaginal lavages, 20/322 (6%) seminal plasmas, and 6/85 (7%) rectal secretions. HIV RNA in DS specimens was near the limit of quantification and not reproducible. HIV DNA was detected in 6/13 (46%) DS cervicovaginal lavages at low levels. Following DNase treatment, 5/39 DS specimens yielded HIV sequences, all without increased genetic distances. Women with and without DS had similar plasma antiretroviral levels and DS in 1 woman was associated with inflammation.CONCLUSIONS:
HIV RNA and DNA sequences and therapeutic antiretroviral plasma levels did not support HIV replication as the cause of DS from the genital tract. Rather, our findings infer that HIV RNA is shed due to proliferation of infected cells with virion production.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
DNA Viral
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RNA Viral
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Infecções por HIV
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HIV-1
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Eliminação de Partículas Virais
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Fármacos Anti-HIV
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Secreções Corporais
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article