Mechanism analysis for the process-dependent driven mode of NaHCO3 in algal antibiotic removal: efficiency, degradation pathway and metabolic response.

ABSTRACT

This work provided a comprehensive perspective to investigate the performance of NaHCO3-driving effect and mechanism including the antibiotic removal, degradation pathway and metabolites analysis, and the algal physiological response during the removal process. Cefuroxime sodium was selected as the target antibiotic. Our results showed that NaHCO3 did not facilitate self-decomposition of the target antibiotic, while drove the improvement on the removal capacity of every algal cell, which then attributed to the total removal efficiency. After 24 h, there was an improvement on the removal rate of the target antibiotic (from 10.21% to 92.89%) when NaHCO3 was added. The degradation pathway of the target antibiotic was confirmed by the formation of three main products (M1, M2 and M3), and the degradation process, that from M1 to M2 and M2 to M3, was accelerated by the existence of NaHCO3. Besides, a 4-stage model illustrated the relationship between NaHCO3 and antibiotic removal process. Moreover, algal culture that supplemented with NaHCO3 demonstrated a better growth capacity. A large increase in the content of chlorophyll a and a moderate increase in the activity of two carbon metabolic enzymes (RuBisCO and CA) might be viewed as a positive response of the algae during the NaHCO3-driving process.