A simple approach to mediate genome editing in the filamentous fungus Trichoderma reesei by CRISPR/Cas9-coupled in vivo gRNA transcription.

Wu, Chuan; Chen, Yumeng; Qiu, Yifei; Niu, Xiao; Zhu, Ningjian; Chen, Jiehui; Yao, Hong; Wang, Wei; Ma, Yushu

Wu, Chuan; Chen, Yumeng; Qiu, Yifei; Niu, Xiao; Zhu, Ningjian; Chen, Jiehui; Yao, Hong; Wang, Wei; Ma, Yushu.

Afiliação

Wu C; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.
Chen Y; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.
Qiu Y; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.
Niu X; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.
Zhu N; The First Affiliated Middle School of East China Normal University, Shanghai, 200086, China.
Chen J; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.
Yao H; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.
Wang W; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China. wadexp@ecust.edu.cn.
Ma Y; State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China. myushu@ecust.edu.cn.

Biotechnol Lett ; 42(7): 1203-1210, 2020 Jul.

Article em En | MEDLINE | ID: mdl-32300998

Assuntos

Sistemas CRISPR-Cas/genética; Edição de Genes/métodos; Genoma Fúngico/genética; Hypocreales/genética; RNA Guia de Cinetoplastídeos/genética; Regiões Promotoras Genéticas/genética; Transcrição Gênica/genética

Palavras-chave

CRISPR/Cas9; Filamentous fungus; Gene editing; In vivo gRNA transcription; Trichoderma reesei

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Genoma Fúngico / RNA Guia de Cinetoplastídeos / Sistemas CRISPR-Cas / Edição de Genes / Hypocreales Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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