Centromere targeting of Mis18 requires the interaction with DNA and H2A-H2B in fission yeast.
Cell Mol Life Sci
; 78(1): 373-384, 2021 Jan.
Article
em En
| MEDLINE
| ID: mdl-32318758
ABSTRACT
Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (spMis18, spMis16 and spMis19) is located to the centromere to license the recruitment of Cnp1CENP-A in Schizosaccharomyces pombe. We found that spMis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A-H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of the extreme C-terminus significantly impairs the localization of spMis18 and Cnp1 to the centromere and delays chromosome segregation during mitosis. Our findings illustrate that the interaction of spMis18 with histone H2A-H2B and DNA plays important roles in the recruitment of spMis18 and Cnp1 to the centromere in fission yeast.
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MEDLINE
Assunto principal:
Schizosaccharomyces
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DNA
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Histonas
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Proteínas de Transporte
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Proteínas de Schizosaccharomyces pombe
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article