Flow cytometric evaluation of TRBC1 expression in tissue specimens and body fluids is a novel and specific method for assessment of T-cell clonality and diagnosis of T-cell neoplasms.
Cytometry B Clin Cytom
; 100(3): 361-369, 2021 05.
Article
em En
| MEDLINE
| ID: mdl-32333725
ABSTRACT
BACKGROUND:
Flow cytometric detection of T-cell clonality is challenging. The current available methodology for T-cell receptor (TCR) Vß repertoire evaluation is a complex assay and has limited sensitivity especially for detecting low levels of disease. Therefore, there is an unmet need for a reliable, simple, and rapid assay to identify T-cell clonality. The rearrangement of the TCRB gene involves the random and mutually exclusive expression of one of two constant ß chain genes (TRBC1 and TRBC2), analogous to the kappa and lambda gene utilization by B cells.METHODS:
Here, we used a single TRBC1 antibody, in conjunction with other T-cell associated markers, to detect T-cell clonality in tissue biopsies and body fluids. A total of 143 tissue/body fluid specimens from 46 patients with a definitive diagnosis of a T-cell neoplasm and 97 patients with no T-cell malignancy were analyzed with a cocktail of monoclonal antibodies including CD2/CD3/CD4/CD5/CD7/CD8/CD45/TCRγδ/TRBC1.RESULTS:
We examined TRBC1 expression on neoplastic T-cell populations identified based on their immunophenotypic aberrancies, and monotypic TRBC1 expression was identified in all 46 known T-cell lymphoma cases. We applied a similar gating strategy to the 97 cases without T-cell neoplasms, and arbitrarily dissected T-cell populations into immunophenotypically distinct subsets; in this group, we found that all cases revealed an expected polytypic TRBC1 expression in all subsets.CONCLUSIONS:
Single TRBC1 antibody detection of T-cell clonality by flow cytometry is a simple, rapid, and robust assay that could be routinely utilized in flow cytometric laboratories.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Líquidos Corporais
/
Linfócitos T
/
Linfoma de Células T
/
Receptores de Antígenos de Linfócitos T alfa-beta
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article