Subtractive Immunization for Mice, Rats, and Hamsters.
Cold Spring Harb Protoc
; 2020(5): 100321, 2020 05 01.
Article
em En
| MEDLINE
| ID: mdl-32358053
ABSTRACT
Subtractive immunization is useful for directing the immune response away from dominant epitopes to allow antibodies against less immunogenic antigens to be developed. It is also useful when there is a need to downplay the response to carriers added to peptides and recombinant proteins, such as Gst and Ig, to aid in production and purification. Subtractive immunization protocols also can be helpful if the target protein is part of a mixture (such as a cell lysate) or present on the surface of transfected cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Imunização
/
Epitopos
/
Antígenos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article