Bioanalytical method validation for the quantification of the chlorogenic acid in Capsicum baccatum through High Performance Liquid Chromatography (HPLC-DAD).

ABSTRACT

The fruit pulp extracts of the species Capsicum baccatum are associated with a number of phenolic compounds. The aim of this study was to validate a bioanalytical method for the quantification of the chlorogenic acid present in the UENF 1613 de C. baccatum accession. The quantification was carried out through HPLC. The results obtained for the extract of C. baccatum indicated selectivity for chlorogenic acid. The values obtained for relative standard deviation, as well as for precision, in terms of repeatability and intermediate precision, did not exceed the maximum of 15% set in the criteria for the acceptance of bioanalytical methods. In terms of recovery, the accuracy rate for the phenolic compound of interest was from 77 to 93%. The validated method through external standardization was applied for the quantification of chlorogenic acid of the UENF accession.