MutaRNA: analysis and visualization of mutation-induced changes in RNA structure.
Nucleic Acids Res
; 48(W1): W287-W291, 2020 07 02.
Article
em En
| MEDLINE
| ID: mdl-32392303
ABSTRACT
RNA molecules fold into complex structures as a result of intramolecular interactions between their nucleotides. The function of many non-coding RNAs and some cis-regulatory elements of messenger RNAs highly depends on their fold. Single-nucleotide variants (SNVs) and other types of mutations can disrupt the native function of an RNA element by altering its base pairing pattern. Identifying the effect of a mutation on an RNA's structure is, therefore, a crucial step in evaluating the impact of mutations on the post-transcriptional regulation and function of RNAs within the cell. Even though a single nucleotide variation can have striking impacts on the structure formation, interpreting and comparing the impact usually needs expertise and meticulous efforts. Here, we present MutaRNA, a web server for visualization and interpretation of mutation-induced changes on the RNA structure in an intuitive and integrative fashion. To this end, probabilities of base pairing and position-wise unpaired probabilities of wildtype and mutated RNA sequences are computed and compared. Differential heatmap-like dot plot representations in combination with circular plots and arc diagrams help to identify local structure abberations, which are otherwise hidden in standard outputs. Eventually, MutaRNA provides a comprehensive and comparative overview of the mutation-induced changes in base pairing potentials and accessibility. The MutaRNA web server is freely available at http//rna.informatik.uni-freiburg.de/MutaRNA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Software
/
RNA
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Mutação
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article