Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.
Commun Biol
; 3(1): 229, 2020 05 11.
Article
em En
| MEDLINE
| ID: mdl-32393743
Primary adult cardiomyocyte (aCM) represent the mature form of myocytes found in the adult heart. However, culture of aCMs in particular is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca2+ cycling to enable physiologically relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations that probe mechanisms underlying myocyte functional performance. Together, these refinements to aCM culture present a toolbox with which to advance our understanding of myocardial physiology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
/
Miócitos Cardíacos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article