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Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.
Callaghan, Neal I; Lee, Shin-Haw; Hadipour-Lakmehsari, Sina; Lee, Xavier A; Ahsan Siraj, M; Driouchi, Amine; Yip, Christopher M; Husain, Mansoor; Simmons, Craig A; Gramolini, Anthony O.
Afiliação
  • Callaghan NI; Institute of Biomaterials and Biomedical Engineering, Faculty of Applied Science and Engineering, University of Toronto, Toronto, ON, Canada.
  • Lee SH; Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, Toronto, ON, Canada.
  • Hadipour-Lakmehsari S; Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, Toronto, ON, Canada.
  • Lee XA; Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Ahsan Siraj M; Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, Toronto, ON, Canada.
  • Driouchi A; Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Yip CM; Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, Toronto, ON, Canada.
  • Husain M; Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
  • Simmons CA; Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
  • Gramolini AO; Ted Rogers Centre for Heart Research, University Health Network, Toronto, Ontario, Canada.
Commun Biol ; 3(1): 229, 2020 05 11.
Article em En | MEDLINE | ID: mdl-32393743
Primary adult cardiomyocyte (aCM) represent the mature form of myocytes found in the adult heart. However, culture of aCMs in particular is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca2+ cycling to enable physiologically relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations that probe mechanisms underlying myocyte functional performance. Together, these refinements to aCM culture present a toolbox with which to advance our understanding of myocardial physiology.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Miócitos Cardíacos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Miócitos Cardíacos Idioma: En Ano de publicação: 2020 Tipo de documento: Article