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A single-cell survey of Drosophila blood.
Tattikota, Sudhir Gopal; Cho, Bumsik; Liu, Yifang; Hu, Yanhui; Barrera, Victor; Steinbaugh, Michael J; Yoon, Sang-Ho; Comjean, Aram; Li, Fangge; Dervis, Franz; Hung, Ruei-Jiun; Nam, Jin-Wu; Ho Sui, Shannan; Shim, Jiwon; Perrimon, Norbert.
Afiliação
  • Tattikota SG; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
  • Cho B; Department of Life Science, Hanyang University, Seoul, Republic of Korea.
  • Liu Y; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
  • Hu Y; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
  • Barrera V; Harvard TH Chan Bioinformatics Core, Boston, United States.
  • Steinbaugh MJ; Harvard TH Chan Bioinformatics Core, Boston, United States.
  • Yoon SH; Department of Life Science, Hanyang University, Seoul, Republic of Korea.
  • Comjean A; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
  • Li F; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
  • Dervis F; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
  • Hung RJ; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
  • Nam JW; Department of Life Science, Hanyang University, Seoul, Republic of Korea.
  • Ho Sui S; Harvard TH Chan Bioinformatics Core, Boston, United States.
  • Shim J; Department of Life Science, Hanyang University, Seoul, Republic of Korea.
  • Perrimon N; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, United States.
Elife ; 92020 05 12.
Article em En | MEDLINE | ID: mdl-32396065
Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Drosophila melanogaster / Hemócitos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Drosophila melanogaster / Hemócitos Idioma: En Ano de publicação: 2020 Tipo de documento: Article