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In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells.
Khatua, Chandra; Min, Sunhong; Jung, Hee Joon; Shin, Jeong Eun; Li, Na; Jun, Indong; Liu, Hui-Wen; Bae, Gunhyu; Choi, Hyojun; Ko, Min Jun; Jeon, Yoo Sang; Kim, Yu Jin; Lee, Joonbum; Ko, Minji; Shim, Gyubo; Shin, Hongchul; Lee, Sangbum; Chung, Seok; Kim, Young Keun; Song, Jae-Jun; Dravid, Vinayak P; Kang, Heemin.
Afiliação
  • Khatua C; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Min S; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Jung HJ; Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, United States.
  • Shin JE; International Institute for Nanotechnology, Evanston, Illinois 60208, United States.
  • Li N; NUANCE Center, Northwestern University, Evanston, Illinois 60208, United States.
  • Jun I; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Liu HW; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul 02841, Republic of Korea.
  • Bae G; Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul 02841, Republic of Korea.
  • Choi H; School of Mechanical Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Ko MJ; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Jeon YS; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Kim YJ; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Lee J; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Ko M; Institute for High Technology Materials and Devices, Korea University, Seoul 02841, Republic of Korea.
  • Shim G; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Shin H; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Lee S; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Chung S; Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea.
  • Kim YK; Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Song JJ; Department of Biomicrosystem Technology, Korea University, Seoul 02841, Republic of Korea.
  • Dravid VP; School of Mechanical Engineering, Korea University, Seoul 02841, Republic of Korea.
  • Kang H; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea.
Nano Lett ; 20(6): 4188-4196, 2020 06 10.
Article em En | MEDLINE | ID: mdl-32406688
Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Células-Tronco / Adesão Celular / Fenômenos Magnéticos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Células-Tronco / Adesão Celular / Fenômenos Magnéticos Idioma: En Ano de publicação: 2020 Tipo de documento: Article