A Simple Apparatus for Individual C. elegans Culture.
Methods Mol Biol
; 2144: 29-45, 2020.
Article
em En
| MEDLINE
| ID: mdl-32410022
ABSTRACT
We miniaturized standard, solid-phase C. elegans culture conditions to produce a system in which many isolated, individual C. elegans can be housed throughout their lives. This system, the "worm corral," is compatible with high-resolution brightfield and fluorescent microscopy, allowing imaging of fluorescent transgenes and morphological phenotypes from hatch until death. These culture devices can be constructed on the benchtop with commercially available reagents and standard laboratory equipment, making this an attainable solution for most labs.
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MEDLINE
Assunto principal:
Caenorhabditis elegans
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Técnicas Analíticas Microfluídicas
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Dispositivos Lab-On-A-Chip
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article