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Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia.
de Campos Facchin, Bruno Matheus; da Rosa, Julia Salvan; Luz, Ana Beatriz Gobbo; Moon, Yeo Jim Kinoshita; de Lima, Tamires Cardoso; Casoti, Rosana; Biavatti, Maique Weber; Dalmarco, Eduardo Monguilhott; Fröde, Tânia Silvia.
Afiliação
  • de Campos Facchin BM; Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
  • da Rosa JS; Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
  • Luz ABG; Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
  • Moon YJK; Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
  • de Lima TC; Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
  • Casoti R; Department of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, SP-, Brazil.
  • Biavatti MW; Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
  • Dalmarco EM; Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
  • Fröde TS; Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, 88040-970 SC-, Brazil.
Mediators Inflamm ; 2020: 4620251, 2020.
Article em En | MEDLINE | ID: mdl-32410853
OBJECTIVE: The aim of this study was to investigate the anti-inflammatory effects of the crude extract (CE), derived fraction, and isolated compounds from Calea pinnatifida leaves in a mouse model of pulmonary neutrophilia. METHODS: The CE and derived fractions, hexane, ethyl acetate, and methanol, were obtained from C. pinnatifida leaves. The compounds 3,5- and 4,5-di-O-E-caffeoylquinic acids were isolated from the EtOAc fraction using chromatography and were identified using infrared spectroscopic data and nuclear magnetic resonance (1H and 13C NMR). Leukocytes count, protein concentration of the exudate, myeloperoxidase (MPO) and adenosine deaminase (ADA), and nitrate/nitrite (NO x ), tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1ß), and interleukin-17A (IL-17A) levels were determined in the pleural fluid leakage after 4 h of pleurisy induction. We also analyzed the effects of isolated compounds on the phosphorylation of both p65 and p38 in the lung tissue. RESULTS: The CE, its fractions, and isolated compounds inhibited leukocyte activation, protein concentration of the exudate, and MPO, ADA, NO x , TNF-α, IL-1ß, and IL-17A levels. 3,5- and 4,5-di-O-E-caffeoylquinic acids also inhibited phosphorylation of both p65 and p38 (P < 0.05). CONCLUSION: This study demonstrated that C. pinnatifida presents important anti-inflammatory properties by inhibiting activated leukocytes and protein concentration of the exudate. These effects were related to the inhibition of proinflammatory mediators. The dicaffeoylquinic acids may be partially responsible for these anti-inflammatory properties through the inhibition of nuclear transcription factor kappa B and mitogen-activated protein kinase pathways.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Asteraceae / Inflamação / Transtornos Leucocíticos / Pneumopatias / Neutrófilos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Asteraceae / Inflamação / Transtornos Leucocíticos / Pneumopatias / Neutrófilos Idioma: En Ano de publicação: 2020 Tipo de documento: Article