Primed CRISPR DNA uptake in Pyrococcus furiosus.
Nucleic Acids Res
; 48(11): 6120-6135, 2020 06 19.
Article
em En
| MEDLINE
| ID: mdl-32421777
CRISPR-Cas adaptive immune systems are used by prokaryotes to defend against invaders like viruses and other mobile genetic elements. Immune memories are stored in the form of 'spacers' which are short DNA sequences that are captured from invaders and added to the CRISPR array during a process called 'adaptation'. Spacers are transcribed and the resulting CRISPR (cr)RNAs assemble with different Cas proteins to form effector complexes that recognize matching nucleic acid and destroy it ('interference'). Adaptation can be 'naïve', i.e. independent of any existing spacer matches, or it can be 'primed', i.e. spurred by the crRNA-mediated detection of a complete or partial match to an invader sequence. Here we show that primed adaptation occurs in Pyrococcus furiosus. Although P. furiosus has three distinct CRISPR-Cas interference systems (I-B, I-A and III-B), only the I-B system and Cas3 were necessary for priming. Cas4, which is important for selection and processing of new spacers in naïve adaptation, was also essential for priming. Loss of either the I-B effector proteins or Cas3 reduced naïve adaptation. However, when Cas3 and all crRNP genes were deleted, uptake of correctly processed spacers was observed, indicating that none of these interference proteins are necessary for naïve adaptation.
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Base de dados:
MEDLINE
Assunto principal:
DNA
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Adaptação Fisiológica
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Pyrococcus furiosus
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Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article