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Prevalence and molecular characterization of Cryptosporidium spp. in Père David's deer (Elaphurus davidianus) in Jiangsu, China.
Huang, Si-Yang; Fan, Yi-Min; Yang, Yi; Ren, Yi-Jun; Gong, Jing-Zhi; Yao, Na; Yang, Bin.
Afiliação
  • Huang SY; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China.
  • Fan YM; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China.
  • Yang Y; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China.
  • Ren YJ; Dafeng Elk National Natural Reserve, Yancheng, Jiangsu Province, PR China.
  • Gong JZ; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China.
  • Yao N; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China.
  • Yang B; College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, PR China.
Rev Bras Parasitol Vet ; 29(2): e017919, 2020.
Article em En | MEDLINE | ID: mdl-32428181
Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David's deer. In this study, 137 fecal samples from Père David's deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in Père David's deer in this area.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Ribossômico / Cervos / DNA de Protozoário / Epidemiologia Molecular / Criptosporidiose / Cryptosporidium Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Ribossômico / Cervos / DNA de Protozoário / Epidemiologia Molecular / Criptosporidiose / Cryptosporidium Idioma: En Ano de publicação: 2020 Tipo de documento: Article