Establishment of homozygous knock-out sea urchins.

Yaguchi, Shunsuke; Yaguchi, Junko; Suzuki, Haruka; Kinjo, Sonoko; Kiyomoto, Masato; Ikeo, Kazuho; Yamamoto, Takashi

Yaguchi, Shunsuke; Yaguchi, Junko; Suzuki, Haruka; Kinjo, Sonoko; Kiyomoto, Masato; Ikeo, Kazuho; Yamamoto, Takashi.

Afiliação

Yaguchi S; Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan; PRESTO, JST, 4-1-8 Honcho, Kawaguchi, 332-0012 Japan. Electronic address: yag@shimoda.tsukuba.ac.jp.
Yaguchi J; Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan.
Suzuki H; Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan.
Kinjo S; Center for Information Biology, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan.
Kiyomoto M; Marine and Coastal Research Center, Ochanomizu University, Kou-yatsu 11, Tateyama, Chiba 294-0301, Japan.
Ikeo K; Center for Information Biology, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan.
Yamamoto T; Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1, Kagamiyama, Higashi-Hiroshima 739-8526, Japan.

Curr Biol ; 30(10): R427-R429, 2020 05 18.

Article em En | MEDLINE | ID: mdl-32428469

ABSTRACT

ABSTRACT

Yaguchi et al. establish a homozygous knock-out sea urchin line by applying the CRISPR-Cas9 system to a new model species, Temnopleurus reevesii, whose breeding cycle takes about half a year.

Assuntos

Sistemas CRISPR-Cas; Técnicas de Inativação de Genes/métodos; Policetídeo Sintases/genética; Ouriços-do-Mar/genética; Animais; Sequência de Bases; DNA/química; DNA/genética; Feminino; Regulação Enzimológica da Expressão Gênica; Homozigoto; Masculino; Mutação

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ouriços-do-Mar / Policetídeo Sintases / Técnicas de Inativação de Genes / Sistemas CRISPR-Cas Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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