Asp isomerization increases aggregation of &#945;-crystallin and decreases its chaperone activity in human lens of various ages.

Fujii, Noriko; Takata, Takumi; Kim, Ingu; Morishima, Ken; Inoue, Rintaro; Magami, Kousuke; Matsubara, Toshiya; Sugiyama, Masaaki; Koide, Tamaki

Asp isomerization increases aggregation of α-crystallin and decreases its chaperone activity in human lens of various ages.

Fujii, Noriko; Takata, Takumi; Kim, Ingu; Morishima, Ken; Inoue, Rintaro; Magami, Kousuke; Matsubara, Toshiya; Sugiyama, Masaaki; Koide, Tamaki.

Afiliação

Fujii N; Institute for Integrated Radiation and Nuclear Science, Kyoto University Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan. Electronic address: nfujii@rri.kyoto-u.ac.jp.
Takata T; Institute for Integrated Radiation and Nuclear Science, Kyoto University Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan.
Kim I; Institute for Integrated Radiation and Nuclear Science, Kyoto University Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan.
Morishima K; Institute for Integrated Radiation and Nuclear Science, Kyoto University Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan.
Inoue R; Institute for Integrated Radiation and Nuclear Science, Kyoto University Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan.
Magami K; Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Matsubara T; Shimadzu Corporation, Sakyo-ku, Kyoto 606-8502, Japan.
Sugiyama M; Institute for Integrated Radiation and Nuclear Science, Kyoto University Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan.
Koide T; Rexxam Co., Ltd., Nishi-ku, Nagoya, Aichi 541-0054, Japan.

Biochim Biophys Acta Proteins Proteom ; 1868(9): 140446, 2020 09.

Article em En | MEDLINE | ID: mdl-32442520

Assuntos

Catarata/metabolismo; Cristalino/química; Agregação Patológica de Proteínas/metabolismo; alfa-Cristalinas/química; Adolescente; Adulto; Idoso; Envelhecimento/metabolismo; Criança; Cromatografia Líquida de Alta Pressão; Cristalinas; Humanos; Isomerismo; Cristalino/metabolismo; Espectrometria de Massas; Pessoa de Meia-Idade; Peso Molecular; Adulto Jovem; alfa-Cristalinas/metabolismo

Palavras-chave

Asp isomers; Chaperone activity; LC-MS-MRM; Lens crystallin; Protein aggregation; Sedimentation coefficient

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Catarata / Alfa-Cristalinas / Agregação Patológica de Proteínas / Cristalino Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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