Promoted off-on recognition of H2O2 based on the fluorescence of silicon quantum dots assembled two-dimensional PEG-MnO2 nanosheets hybrid nanoprobe.

ABSTRACT

An "off-on" assay system for H2O2 determination was developed based on assembling ultra-bright fluorescent silicon quantum dots (SiQDs) and PEG-MnO2 nanosheets. Among them, SiQDs acted as fluorometric reporter, which can effectively eliminate the interference of plant pigments under excitation of 365 nm. PEG-MnO2 nanosheets played dual function of nanoquencher and H2O2 recognizer. Unlike previous reports, the quenching mechanism of SiQDs by PEG-MnO2 nanosheets is attributed to both the associative effect of inner filter effect and the static quenching effect. Thus, the fluorescence intensity of SiQDs at 445 nm decreased with increasing concentration of PEG-MnO2 nanosheets. After addition of H2O2, PEG-MnO2 nanosheets were reduced to Mn2+, consequently resulting in the recovery of the SiQDs fluorescence. Combined with these properties, an off-on fluorescent method was built for determination of H2O2 in plant leaves with high sensitivity and selectivity. The present method has two linear ranges from 0.05 to 1 µM with a detection limit of 0.09 µM and from 1 to 80 µM with a detection limit of 4.04 µM. Graphical abstract Schematic representation of the mechanism of SiQD/PEG-MnO2 nanoprobe for determination of H2O2.