MicroRNA103a3p promotes metastasis by targeting TPD52 in salivary adenoid cystic carcinoma.
Int J Oncol
; 57(2): 574-586, 2020 08.
Article
em En
| MEDLINE
| ID: mdl-32467999
ABSTRACT
Salivary adenoid cystic carcinoma (SACC) exhibits slow continuous growth, frequent local recurrences and a high incidence of blood metastasis, with advanced lung metastasis frequently occurring and being among the primary causes of mortality. MicroRNAs (miR) serve a significant role in the initiation and development of cancer and may be tumourspecific molecular targets. However, the role of miR103a3p in SACC remains largely unknown. In the present study, the expression levels of miR103a3p and tumour protein D52 (TPD52) were detected by reverse transcriptionquantitative PCR. In addition, woundhealing assays, Transwell assays and mouse models of lung metastasis were used to investigate the biological functions exerted by miR103a3p. The present results suggested that miR103a3p expression was significantly upregulated in SACC samples. Gainoffunction and lossoffunction studies in SACC cells demonstrated that miR103a3p acted as an oncogene by promoting tumour cell migration in vitro and lung metastasis in vivo. Dualluciferase reporter gene assays indicated that miR103a3p exerted its regulatory functions by binding to the 3' untranslated region of TPD52 mRNA. TPD52 overexpression rescued the effect of miR103a3p on promoting SACC cell migration, suggesting that miR103a3p acted as an oncogene to promote cancer metastasis by directly targeting TPD52. Thus, the newly identified miR103a3p/TPD52 axis contributes to the understanding of SACC pathogenesis, providing insights into the identification of novel biomarkers or potential therapeutic targets in SACC.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias das Glândulas Salivares
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Carcinoma Adenoide Cístico
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MicroRNAs
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Neoplasias Pulmonares
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Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article