A requirement for an active proton delivery network supports a compound I-mediated C-C bond cleavage in CYP51 catalysis.

Hargrove, Tatiana Y; Wawrzak, Zdzislaw; Guengerich, F Peter; Lepesheva, Galina I

Hargrove, Tatiana Y; Wawrzak, Zdzislaw; Guengerich, F Peter; Lepesheva, Galina I.

Afiliação

Hargrove TY; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Wawrzak Z; Synchrotron Research Center, Life Science Collaborative Access Team, Northwestern University, Argonne, Illinois, USA.
Guengerich FP; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Lepesheva GI; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA galina.i.lepesheva@vanderbilt.edu.

J Biol Chem ; 295(29): 9998-10007, 2020 07 17.

Article em En | MEDLINE | ID: mdl-32493730

Assuntos

Prótons; Esterol 14-Desmetilase/química; Aromatase/química; Catálise; Enzima de Clivagem da Cadeia Lateral do Colesterol/química; Cristalografia por Raios X; Humanos

Palavras-chave

CYP51; CC bond cleavage; X-ray crystallography; X-ray structure; compound I; conformational change; cytochrome P450; cytochrome P450 mechanism; deformylation; enzyme catalysis; enzyme mechanism; hydroxylation; proton relay; sterol 14α-demethylase; sterol biosynthesis; structural biology

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Prótons / Esterol 14-Desmetilase Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Prótons / Esterol 14-Desmetilase Idioma: En Ano de publicação: 2020 Tipo de documento: Article