Correlation analysis between C natriuretic peptide and pregnancy outcome.

ABSTRACT

C-type natriuretic peptide (CNP) plays a key role in female reproduction and is related to oocyte quality. This study analyzed the relationship of CNP with pregnancy outcome to provide a new indicator of pregnancy outcome. Follicular fluids were collected from 158 patients undergoing the IVF/ICSI procedure at the Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology. CNP and cGMP levels in human follicular fluids were tested by ELISA. Then, the distribution patterns of CNP and NPR-B from GV oocytes to blastocysts in mice were tested by confocal microcopy. Finally, CNP was added to the fertility or embryo development medium to observe fertility rate and the development of the embryo. CNP levels in follicular fluids from nonpregnant women were significantly higher than those in follicular fluids from pregnant women. A strong positive correlation between CNP and cGMP concentrations in human follicular fluids was observed. Both CNP and NPR-B were expressed in the plasma of cells at different stages from GV to blastocyst. CNP could increase the 2-cell rate of embryos and the rate of blastocysts when added to either fertility culture medium or embryo culture medium. In a word, CNP in human follicular fluid could predict the pregnancy outcome of IVF patients, and the mechanism of CNP in follicular fluid is related to the quality of oocyte or embryo competence and could promote the development of embryos.