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Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay.
Broughton, James P; Deng, Xianding; Yu, Guixia; Fasching, Clare L; Singh, Jasmeet; Streithorst, Jessica; Granados, Andrea; Sotomayor-Gonzalez, Alicia; Zorn, Kelsey; Gopez, Allan; Hsu, Elaine; Gu, Wei; Miller, Steve; Pan, Chao-Yang; Guevara, Hugo; Wadford, Debra A; Chen, Janice S; Chiu, Charles Y.
Afiliação
  • Broughton JP; Mammoth Biosciences, Inc., San Francisco, California, USA.
  • Deng X; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Yu G; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California, USA.
  • Fasching CL; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Singh J; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California, USA.
  • Streithorst J; Mammoth Biosciences, Inc., San Francisco, California, USA.
  • Granados A; Mammoth Biosciences, Inc., San Francisco, California, USA.
  • Sotomayor-Gonzalez A; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Zorn K; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Gopez A; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California, USA.
  • Hsu E; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Gu W; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California, USA.
  • Miller S; Department of Biochemistry and Biophysics, San Francisco, California, USA.
  • Pan CY; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Guevara H; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Wadford DA; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Chen JS; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Chiu CY; Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA, USA.
medRxiv ; 2020 Mar 27.
Article em En | MEDLINE | ID: mdl-32511449
ABSTRACT
An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2 DETECTR, a rapid (~30 min), low-cost, and accurate CRISPR-Cas12 based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated this method using contrived reference samples and clinical samples from infected US patients and demonstrated comparable performance to the US CDC SARS-CoV-2 real-time RT-PCR assay.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article