Human immunoglobulin G responses to Cimex lectularius L. saliva.
Parasite Immunol
; 42(12): e12764, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-32516446
ABSTRACT
AIMS:
To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L. METHODS ANDRESULTS:
Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract.CONCLUSIONS:
Human participants did not have consistent IgG responses to crushed C lectularius salivary gland extract.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saliva
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Percevejos-de-Cama
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Imunoglobulina G
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Mordeduras e Picadas de Insetos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article