Genomic and signalling pathway characterization of the NZM panel of melanoma cell lines: A valuable model for studying the impact of genetic diversity in melanoma.
Pigment Cell Melanoma Res
; 34(1): 136-143, 2021 01.
Article
em En
| MEDLINE
| ID: mdl-32567790
ABSTRACT
Melanoma is a disease associated with a very high mutation burden and thus the possibility of a diverse range of oncogenic mechanisms that allow it to evade therapeutic interventions and the immune system. Here, we describe the characterization of a panel of 102 cell lines from metastatic melanomas (the NZM lines), including using whole-exome and RNA sequencing to analyse genetic variants and gene expression changes in a subset of this panel. Lines possessing all major melanoma genotypes were identified, and hierarchical clustering of gene expression profiles revealed four broad subgroups of cell lines. Immunogenotyping identified a range of HLA haplotypes as well as expression of neoantigens and cancer-testis antigens in the lines. Together, these characteristics make the NZM panel a valuable resource for cell-based, immunological and xenograft studies to better understand the diversity of melanoma biology and the responses of melanoma to therapeutic interventions.
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Assunto principal:
Biomarcadores Tumorais
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Regulação Neoplásica da Expressão Gênica
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Genômica
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Exoma
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Melanoma
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Modelos Biológicos
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Mutação
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article