Ischemic Preconditioning Protects Against Hepatic Ischemia-Reperfusion Injury Under Propofol Anesthesia in Rats.
Transplant Proc
; 52(10): 2964-2969, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-32586662
ABSTRACT
BACKGROUND:
Propofol is widely used in general anesthesia, and it has been reported to protect various organs against ischemia-reperfusion injury (IRI), including liver. To evaluate the hepatoprotective effects of ischemic preconditioning (IP) under propofol anesthesia, we investigated the possible underlying mechanisms in rats.METHODS:
Male Sprague-Dawley rats were randomly assigned to 3 groups sham group (n = 5), non-IP group (n = 9; 45 minutes of hepatic ischemia followed by 2 hours of reperfusion), and IP group (n = 9; IP applied as 10 minutes of hepatic ischemia followed by 15 minutes of reperfusion before 45 minutes of ischemia). Anesthesia was maintained with intravenous (IV) infusion of propofol (800 µg/kg/min). Liver enzymes, histopathological changes, and cytokine expression were examined.RESULTS:
The IP group showed significantly lower liver enzyme levels (aspartate aminotransferase, P = .045; alanine aminotransferase, P = .006) and reduced the histologic grades of hepatic injury 2 hours after reperfusion (P = .004) compared to the non-IP group. Lactate dehydrogenase activity (P < .001) and interleukin-6 mRNA levels were significantly higher in the non-IP group than in the sham and IP groups (P = .002, both groups).CONCLUSIONS:
Our results demonstrate that IP under propofol anesthesia significantly attenuated hepatic IRI. The principal mechanism of the protective effects appeared to involve reduced expression of the IL-6 pro-inflammatory cytokine and subsequent reduction of the degree of necrosis.
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Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
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Propofol
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Anestésicos Intravenosos
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Precondicionamento Isquêmico
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Fígado
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article