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Male reproductive aging arises via multifaceted mating-dependent sperm and seminal proteome declines, but is postponable in Drosophila.
Sepil, Irem; Hopkins, Ben R; Dean, Rebecca; Bath, Eleanor; Friedman, Solomon; Swanson, Ben; Ostridge, Harrison J; Harper, Lucy; Buehner, Norene A; Wolfner, Mariana F; Konietzny, Rebecca; Thézénas, Marie-Laëtitia; Sandham, Elizabeth; Charles, Philip D; Fischer, Roman; Steinhauer, Josefa; Kessler, Benedikt M; Wigby, Stuart.
Afiliação
  • Sepil I; Department of Zoology, University of Oxford, OX1 3SZ Oxford, United Kingdom; irem.sepil@zoo.ox.ac.uk.
  • Hopkins BR; Department of Zoology, University of Oxford, OX1 3SZ Oxford, United Kingdom.
  • Dean R; Department of Ecology and Evolution, University of California, Davis, CA 95616.
  • Bath E; Department of Genetics, Evolution and Environment, University College London, WC1E 6BT London, United Kingdom.
  • Friedman S; Department of Zoology, University of Oxford, OX1 3SZ Oxford, United Kingdom.
  • Swanson B; Department of Biology, Yeshiva University, New York, NY 10033.
  • Ostridge HJ; Department of Zoology, University of Oxford, OX1 3SZ Oxford, United Kingdom.
  • Harper L; Department of Zoology, University of Oxford, OX1 3SZ Oxford, United Kingdom.
  • Buehner NA; Department of Genetics, Evolution and Environment, University College London, WC1E 6BT London, United Kingdom.
  • Wolfner MF; Department of Zoology, University of Oxford, OX1 3SZ Oxford, United Kingdom.
  • Konietzny R; School of Biology, University of St Andrews, KY16 9ST St Andrews, United Kingdom.
  • Thézénas ML; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853.
  • Sandham E; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853.
  • Charles PD; Nuffield Department of Medicine, TDI Mass Spectrometry Laboratory, Target Discovery Institute, University of Oxford, OX3 7FZ Oxford, United Kingdom.
  • Fischer R; Nuffield Department of Medicine, TDI Mass Spectrometry Laboratory, Target Discovery Institute, University of Oxford, OX3 7FZ Oxford, United Kingdom.
  • Steinhauer J; Department of Zoology, University of Oxford, OX1 3SZ Oxford, United Kingdom.
  • Kessler BM; Nuffield Department of Medicine, TDI Mass Spectrometry Laboratory, Target Discovery Institute, University of Oxford, OX3 7FZ Oxford, United Kingdom.
  • Wigby S; Nuffield Department of Medicine, TDI Mass Spectrometry Laboratory, Target Discovery Institute, University of Oxford, OX3 7FZ Oxford, United Kingdom.
Proc Natl Acad Sci U S A ; 117(29): 17094-17103, 2020 07 21.
Article em En | MEDLINE | ID: mdl-32611817
Declining ejaculate performance with male age is taxonomically widespread and has broad fitness consequences. Ejaculate success requires fully functional germline (sperm) and soma (seminal fluid) components. However, some aging theories predict that resources should be preferentially diverted to the germline at the expense of the soma, suggesting differential impacts of aging on sperm and seminal fluid and trade-offs between them or, more broadly, between reproduction and lifespan. While harmful effects of male age on sperm are well known, we do not know how much seminal fluid deteriorates in comparison. Moreover, given the predicted trade-offs, it remains unclear whether systemic lifespan-extending interventions could ameliorate the declining performance of the ejaculate as a whole. Here, we address these problems using Drosophila melanogaster. We demonstrate that seminal fluid deterioration contributes to male reproductive decline via mating-dependent mechanisms that include posttranslational modifications to seminal proteins and altered seminal proteome composition and transfer. Additionally, we find that sperm production declines chronologically with age, invariant to mating activity such that older multiply mated males become infertile principally via reduced sperm transfer and viability. Our data, therefore, support the idea that both germline and soma components of the ejaculate contribute to male reproductive aging but reveal a mismatch in their aging patterns. Our data do not generally support the idea that the germline is prioritized over soma, at least, within the ejaculate. Moreover, we find that lifespan-extending systemic down-regulation of insulin signaling results in improved late-life ejaculate performance, indicating simultaneous amelioration of both somatic and reproductive aging.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatozoides / Envelhecimento / Proteínas de Plasma Seminal / Drosophila melanogaster Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatozoides / Envelhecimento / Proteínas de Plasma Seminal / Drosophila melanogaster Idioma: En Ano de publicação: 2020 Tipo de documento: Article