Construction of an Efficient Nicotinate Dehydrogenase Expression System in Comamonas testosteroni CNB-2 with Multi-level N-Terminal Engineering.

Lu, Zhen-Hua; Yang, Li-Rong; Wu, Jian-Ping

Lu, Zhen-Hua; Yang, Li-Rong; Wu, Jian-Ping.

Afiliação

Lu ZH; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
Yang LR; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
Wu JP; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China. wjp@zju.edu.cn.

Appl Biochem Biotechnol ; 192(3): 923-934, 2020 Nov.

Article em En | MEDLINE | ID: mdl-32617841

Assuntos

Comamonas testosteroni/genética; Engenharia Genética; Oxirredutases atuantes sobre Doadores de Grupo CH-NH/química; Oxirredutases atuantes sobre Doadores de Grupo CH-NH/genética; Genes Reporter/genética; Regiões Promotoras Genéticas/genética

Palavras-chave

Balanced expression; N-terminal engineering; Nicotinate dehydrogenase; Promoter engineering; SD sequence modification; SKIK-like strategy

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Engenharia Genética / Comamonas testosteroni / Oxirredutases atuantes sobre Doadores de Grupo CH-NH Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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