TGF-ß3 suppresses melanogenesis in human melanocytes cocultured with UV-irradiated neighboring cells and human skin.
J Dermatol Sci
; 99(2): 100-108, 2020 Aug.
Article
em En
| MEDLINE
| ID: mdl-32620316
ABSTRACT
BACKGROUND:
Ultraviolet radiation (UVR) is the most well-known cause of skin pigmentation accompanied with photoaging. Transforming growth factor (TGF)-ß1 was previously shown to have anti-melanogenic property; however, it can induce scarring in skin.OBJECTIVE:
We investigated the effect of TGF-ß3 on melanogenesis in human melanocytes cocultured with UV-irradiated skin constituent cells, and UV-irradiated human skin.METHODS:
UVB irradiation or treatment with stem cell factor (SCF) and endothelin-1 (ET-1) was applied to human melanocytes cocultured with keratinocytes and/or fibroblasts and ex vivo human skin. Mechanistic pathways were further explored after treatment with TGF-ß3.RESULTS:
While UVB irradiation or SCF/ET-1 enhanced melanogenesis, TGF-ß3 effectively inhibited melanin accumulation and tyrosinase activity via downregulation of the extracellular signal-regulated kinase (ERK)/microphthalmia-associated transcription factor (MITF) pathway. TGF-ß3 increased the expression of differentiation markers of keratinocytes.CONCLUSION:
TGF-ß3 effectively suppressed UVR-stimulated melanogenesis indicating that topical TGF-ß3 may be a suitable candidate for the treatment of UV-associated hyperpigmentation disorders.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Pele
/
Raios Ultravioleta
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Fator de Crescimento Transformador beta3
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Melaninas
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Melanócitos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article