High resolution single particle Cryo-EM refinement using JSPR.
Prog Biophys Mol Biol
; 160: 37-42, 2021 03.
Article
em En
| MEDLINE
| ID: mdl-32622834
ABSTRACT
JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review, we will present examples achieved using JSPR and demonstrate recently implemented features of JSPR such as multi-aberration "alignments" and automatic optimization of masking for the assessment of map resolution using "true" FSC.
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MEDLINE
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Vírus
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Microscopia Crioeletrônica
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Imagem Individual de Molécula
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article