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MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1.
Kornuta, Claudia A; Bidart, Juan E; Soria, Ivana; Gammella, Mariela; Quattrocchi, Valeria; Pappalardo, Juan S; Salmaso, Stefano; Torchilin, Vladimir P; Cheuquepán Valenzuela, Felipe; Hecker, Yanina P; Moore, Dadin P; Zamorano, Patricia I; Langellotti, Cecilia A.
Afiliação
  • Kornuta CA; Instituto de Virología e Innovaciones Tecnológicas (IVIT, INTA-CONICET), Hurlingham, Argentina.
  • Bidart JE; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
  • Soria I; Instituto de Virología e Innovaciones Tecnológicas (IVIT, INTA-CONICET), Hurlingham, Argentina.
  • Gammella M; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
  • Quattrocchi V; Instituto de Virología e Innovaciones Tecnológicas (IVIT, INTA-CONICET), Hurlingham, Argentina.
  • Pappalardo JS; Instituto de Virología e Innovaciones Tecnológicas (IVIT, INTA-CONICET), Hurlingham, Argentina.
  • Salmaso S; Instituto de Virología e Innovaciones Tecnológicas (IVIT, INTA-CONICET), Hurlingham, Argentina.
  • Torchilin VP; Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB, INTA-CONICET), Río Negro, Argentina.
  • Cheuquepán Valenzuela F; Department of Pharmaceutical and Pharmacological Sciences, Universita degli Studi di Padova, Padova PD, Italy.
  • Hecker YP; Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA.
  • Moore DP; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
  • Zamorano PI; EEA Balcarce, Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce, Buenos Aires, Argentina.
  • Langellotti CA; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
Transbound Emerg Dis ; 68(2): 587-597, 2021 Mar.
Article em En | MEDLINE | ID: mdl-32643286
New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus-1 (BoHV-1) infection have several disadvantages. Previous works on DNA vaccines against BoHV-1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, 'naked' DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen-presenting cells (APC). In this work, a DNA-based vaccine encoding the truncated version of BoHV-1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1-2MAN-PEG-DOPE (pCIgD-Man-L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD-Man-L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV-1 was detected in animals vaccinated with pCIgD-Man-L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1-2MAN-PEG-DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1-2MAN decorated liposomes can boost the immunogenicity resulting in a long-lasting immunity. Liposomes decorated with MANα1-2MAN-PEG-DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV-1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças dos Bovinos / Vacinação / Infecções por Herpesviridae / Herpesvirus Bovino 1 / Vacinas contra Herpesvirus Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças dos Bovinos / Vacinação / Infecções por Herpesviridae / Herpesvirus Bovino 1 / Vacinas contra Herpesvirus Idioma: En Ano de publicação: 2021 Tipo de documento: Article