Activation of Caspase-3 by Terpenoids and Flavonoids in Different Types of Cancer Cells.
Curr Top Med Chem
; 20(21): 1876-1887, 2020.
Article
em En
| MEDLINE
| ID: mdl-32648841
ABSTRACT
BACKGROUND:
Caspase-3 is accountable for the execution of apoptosis. Recently, it has gained attention as a promising target for the discovery of natural products as anticancer agents.METHODS:
We examined the efficacy of two different sets of natural products (terpenoids and flavonoids) towards caspase-3 activity adopting in silico, cell-free and cell-based activity and real-time gene expression analysis.RESULTS:
It was observed that terpenes activate caspase-3 activity in both the cell-free and cell-based systems, which was supported by the gene expression analysis, binding energy and activation constant. Flavonoids' action, however, was limited to the cell-based system and transcriptional regulation suggesting their indirect association, which enhanced the enzyme activity and up-regulated the expression of mRNA levels in the cells. Among the tested natural products, (+) carvone was observed to be the best activator of caspase-3 in K562 (34.4 µM), WRL-68 (22.3 µM), HeLa (18.7 µM), MCF-7 (39.4 µM) and MDA-MB-231 cell lines (45.1 µM).CONCLUSION:
Overall, terpenoids have a persistent activation of caspase-3 in all the investigated systems, while flavonoids circuitously affect the enzyme activity.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Terpenos
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Flavonoides
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Produtos Biológicos
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Caspase 3
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Antineoplásicos Fitogênicos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article