Improving the biocatalytic performance of co-immobilized cells harboring nitrilase via addition of silica and calcium carbonate.
Bioprocess Biosyst Eng
; 43(12): 2201-2207, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-32661565
ABSTRACT
To improve nicotinic acid (NA) yield and meet industrial application requirements of sodium alginate-polyvinyl alcohol (SA-PVA) immobilized cells of Pseudomonas putida mut-D3 harboring nitrilase, inorganic materials were added to the SA-PVA immobilized cells to improve mechanical strength and mass transfer performance. The concentrations of inorganic materials were optimized to be 2.0% silica and 0.6% CaCO3. The optimal pH and temperature for SA-PVA immobilized cells and composite immobilized cells were both 8.0 and 45 °C, respectively. The half-lives of composite immobilized cells were 271.48, 150.92, 92.92 and 33.12 h, which were 1.40-, 1.35-, 1.22- and 1.63-fold compared to SA-PVA immobilized cells, respectively. The storage stability of the composite immobilized cells was slightly increased. The composite immobilized cells could convert 14 batches of 3-cyanopyridine with feeding concentration of 250 mM and accumulate 418 g ·L-1 nicotinic acid, while the SA-PVA immobilized cells accumulated 346 g L-1 nicotinic acid.
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Base de dados:
MEDLINE
Assunto principal:
Álcool de Polivinil
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Pseudomonas putida
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Alginatos
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Aminoidrolases
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article