Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9.
BMC Microbiol
; 20(1): 217, 2020 07 20.
Article
em En
| MEDLINE
| ID: mdl-32689952
ABSTRACT
BACKGROUND:
Lactobacillus gasseri OLL2809 can highly induce interleukin (IL)-12 production in immune cells. Even though beneficial properties of this strain for both humans and animals have been reported, the mechanism by which the bacteria induces the production of IL-12 in immune cells remains elusive. In this study, we investigated the mechanism of induction of IL-12 using a mouse macrophage cell line J774.1.RESULTS:
Inhibition of phagocytosis of L. gasseri OLL2809, and myeloid differentiation factor 88 and Toll-like receptors (TLRs) 7 and 9 signalling attenuated IL-12 production in J774.1 cells. Total RNA and genomic DNA of L. gasseri OLL2809, when transferred to the J774.1 cells, also induced IL-12 production. The difference in the IL-12-inducing activity of Lactobacilli is attributed to the susceptibility to phagocytosis, but not to a difference in the total RNA and genomic DNA of each strain.CONCLUSION:
We concluded that total RNA and genomic DNA of phagocytosed L. gasseri OLL2809 induce IL-12 production in J774.1 cell via TLRs 7 and 9, and the high IL-12-inducing activity of L. gasseri OLL2809 is due to its greater susceptibility to phagocytosis.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
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RNA Bacteriano
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Glicoproteínas de Membrana
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Interleucina-12
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Receptor Toll-Like 9
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Receptor 7 Toll-Like
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Lactobacillus gasseri
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Macrófagos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article